Repolarization studies using human stem cell-derived cardiomyocytes: Validation studies and best practice recommendations
نویسندگان
چکیده
منابع مشابه
Human-induced pluripotent stem cell-derived cardiomyocytes for studies of cardiac ion transporters.
Human-induced pluripotent stem cells (hiPSCs) can differentiate into functional cardiomyocytes (iCell Cardiomyocytes) with ion channel activities that are remarkably similar to adult cardiomyocytes. Here, we extend this characterization to cardiac ion transporters. Additionally, we document facile molecular biological manipulation of iCell Cardiomyocytes to overexpress and knockdown transporter...
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The discovery of the genetic basis of inherited arrhythmias has paved the way for an improved understanding of arrhythmogenesis in a wide spectrum of life-threatening conditions. In vitro expression of mutations and transgenic animal models have been instrumental in enhancing this understanding, but the applicability of results to the human heart remains unknown. The ability to differentiate in...
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in spite of serious cardiotoxicity side-effects, doxorubicin is frequently used for treatment of several types of cancers. isolated human adult cardiomyocytes could be the best model for assessing drug-induced cardiotoxicity, while harvesting mature cardiomyocytes is restricted by some limitations such as biopsy size, cell numbers, viability, proliferative capacity and their disability to be pa...
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Long QT syndrome (LQTS) is characterized by a prolonged QT-interval on electrocardiogram and by increased risk of sudden death. One of the most common and potentially life-threatening electrolyte disturbances is hypokalemia, characterized by low concentrations of K+ Using a multielectrode array platform and current clamp technique, we investigated the effect of low extracellular K+ concentratio...
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In the past 6 years, multiple laboratories have reported the successful directed cardiomyocyte differentiation from human pluripotent stem cells (hPSCs; reviewed in Burridge et al). These stem cell–derived cardiomyocytes beat spontaneously, express the expected sarcomeric proteins and ion channels, and exhibit cardiac-type action potentials and calcium transients. They show similar functional p...
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ژورنال
عنوان ژورنال: Regulatory Toxicology and Pharmacology
سال: 2020
ISSN: 0273-2300
DOI: 10.1016/j.yrtph.2020.104756